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KMID : 0380020080230050445
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 5 p.445 ~ p.448
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Functional expression of CalB in E.coli
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Abstract
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Candida antarctica lipase B (CalB) is an efficient biocatalyst for many organic synthesis reactions. To make full use of CalB, we need effective expression system. Previously recom binant CalB was successfully expressed in the methylotropic yeast Pichia pastoris. In addition, we succeed in the functional expression of CalB in the Escherichia coli cytoplasm. This CalB expression system in E.coli has many considerable advantages in comparison with other
expression systems and enables high-throughput screening of gene libraries as those derived from directed evolution experiments. To optimize E.coli system, we investigate comparing between OrigamiB (DE3) and BL21 (DE3) and observing effect of IPTG amount.
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KEYWORD
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Lipase, Candida antarctica lipase, OrigamiB, cold-shock promoter
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